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Microclimatic conditions as determinant of Vaccinium myrtillus pollination
Veljačiková, Hana ; Janovský, Zdeněk (advisor) ; Červenková, Zita (referee)
Climate change affects phenology and distribution of plants and animals and causes a mismatch in plant-pollinator interactions. These changes are clearly manifested in extreme environments such as mountains or the beginning of the growing season. Vaccinium myrtillus is an important temperate and boreal species, inhabiting and often dominating such environments where changes due to global warming are already appearing. Flowers start to appear in April and May at the very beginning of the growing season. Without pollination, flower will almost never form a fruit. When comparing fruit set, it is possible to observe whether the flower has been pollinated and thus the quality of pollination in given conditions. Using experiments on the mountain heath in the Brdy Protected Land Area, I found out how the quality of pollination differs in different microclimatic conditions. I compared fruit set of chosen flowers and seed set in their fruits. Flowers blooming at the end of the flowering season had the greatest success in fruit formation, when I recorded a greater number of pollinators in the experimental environment. V. myrtillus bloomed more intensively at the beginning of the flowering season. Even so, fewer fruits were produced from flowers at that time. Bumblebees abundance was correlated to fruit and...
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Population genetics and speciation in Stylops (Strepsiptera)
Kodejš, Karel ; Straka, Jakub (advisor) ; Černá, Kateřina (referee)
This thesis is focused to population genetics of selected species of genus Stylops in Europe. Within this genus, five species was analysed by usage of two approaches - microsatellite analysis in Stylops ater and analysis of mitochondrial DNA in all five species. Mitochondrial gene for cytochome c oxidase (1st subunit) was used. For microsatellite analysis was performed bayesian clustering analysis and ABC approach (Aproximate Bayesian Computation). Mitochondrial markers were processed by making haplotype networks and demography analysis by computing Bayesian skyline plots. For Stylops ater, surprisingly low lewel of population subdivision was detected, yet with clearly differentiated population clusters from Scandinavia and baltic coast of Europe, which may imply period of isolation of these populations or relativelly recent population expansion and genetic differentiation due to lower population sizes. Next, hypotesis of possible temporal segregation of subpopulations of Stylops nevinsoni based on different aktivity period of their host species groups, was supported. In other three species, population subdivision was observed to be related either due to host specialisation (Stylops mellitae) or geographic consequences (S.nassonowi, S.spreta). Interesting finding is also detection of population...
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